equilibrating buffer

Greek translation: Ρυθμιστικό διάλυμα εξισορρόπησης

09:57 Mar 30, 2011

English to Greek translations [PRO]
Science - Food & Drink / microbiology, chemical reactions

English term or phrase: equilibrating buffer

For fractioning of aminopeptidase activities, dialysed cheese or micro-organism extracts were submitted to batch chromatography in Q-Sepharose (Amersham), equilibrated in 20 mM sodium phosphate, pH 7.5, in a proportion of one A280 unit of protein per mL packed resin. Samples and resin were mixed in Becker and kept under stirring in an ice bath for 2 h. The mixture was poured into a glass-sintered funnel and washed with the equilibrating buffer to recover nonretained fractions. Three elution steps were carried out, adding NaCl to the equilibrating buffer: fraction I: 150 mM NaCl; fraction II: 300 mM NaCl; and fraction III 500 mM NaCl. The protein content and aminopeptidase activity of fractions were analysed as described.

Kyriacos Georghiou KudoZ activity Questions: 33 (1 open) (1 without valid answers) (1 closed without grading) Answers: 709 Cyprus
Local time: 12:03

Greek translation:Ρυθμιστικό διάλυμα εξισορρόπησης

Explanation:
Ρυθμιστικό διάλυμα εξισορρόπησης = Equilibration buffer

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Note added at 14 mins (2011-03-30 10:12:19 GMT)
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Selected response from:

Vicky Valla
Local time: 12:03

Grading comment

Thank you V.
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Ρυθμιστικό διάλυμα εξισορρόπησης

Vicky Valla

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Ρυθμιστικό διάλυμα εξισορρόπησης

Vicky Valla
Local time: 12:03
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PRO pts in category: 4

Grading comment

Thank you V.

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equilibrating buffer

Greek translation: Ρυθμιστικό διάλυμα εξισορρόπησης

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equilibrating buffer

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