whole mount staining

Portuguese translation: coloração com montagem total

12:43 Jan 22, 2018
English to Portuguese translations [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: whole mount staining
"For whole mount CP staining, tissues were fixated with 2.5% paraformaldehyde (PFA) for 1 hour at 4°C, and subsequently transferred to PBS containing 0.05% sodium azide. Prior to staining, the dissected tissues were washed with PBS and blocked (20% horse serum, 0.3% Triton X-100, and PBS) for lh at room temperature.Whole mount staining with primary antibodies (in PBS containing 2% horse serum and 0.3% Triton X-100), or secondary antibodies, was performed for 1h at room temperature."
Isadora Vital
United States
Portuguese translation:coloração com montagem total
Explanation:
Dra Maria Izabel...
http://www.biologia.ufc.br/backup/docentes/IzabelGallao/Grad...
Montagem total
Ex: túbulo de Malpighi, glândulas salivares


https://biollogy.wordpress.com/biologia-celular/
Os cortes possíveis para a análise...
montagem total ———> órgãos...
Selected response from:

Matheus Chaud
Brazil
Local time: 12:06
Grading comment
Selected automatically based on peer agreement.
4 KudoZ points were awarded for this answer



Summary of answers provided
3 +4coloração com montagem total
Matheus Chaud
4 +1coloração da montagem total
Leandro Borges


  

Answers


7 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5 peer agreement (net): +4
whole mount staining
coloração com montagem total


Explanation:
Dra Maria Izabel...
http://www.biologia.ufc.br/backup/docentes/IzabelGallao/Grad...
Montagem total
Ex: túbulo de Malpighi, glândulas salivares


https://biollogy.wordpress.com/biologia-celular/
Os cortes possíveis para a análise...
montagem total ———> órgãos...


Matheus Chaud
Brazil
Local time: 12:06
Native speaker of: Native in PortuguesePortuguese
PRO pts in category: 120
Grading comment
Selected automatically based on peer agreement.

Peer comments on this answer (and responses from the answerer)
agree  Stagi (X)
47 mins
  -> Obrigado, Stagi!

agree  Mario Freitas:
8 hrs
  -> Obrigado, Mário!

agree  Paulo Gasques
16 hrs
  -> Obrigado, Paulo!

agree  Margarida Ataide
1 day 1 hr
  -> Obrigado, Margarida!
Login to enter a peer comment (or grade)

1 hr   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +1
coloração da montagem total


Explanation:
Conforme o contexto:
"Para a coloração da montagem total do CP..."

whole mount: montagem/preparação total

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031...

Leandro Borges
Brazil
Local time: 12:06
Specializes in field
Native speaker of: Native in PortuguesePortuguese
PRO pts in category: 12

Peer comments on this answer (and responses from the answerer)
agree  Jonatas Ribeiro de Andrade
16 mins
  -> obrigado, Jonatas!
Login to enter a peer comment (or grade)



Login or register (free and only takes a few minutes) to participate in this question.

You will also have access to many other tools and opportunities designed for those who have language-related jobs (or are passionate about them). Participation is free and the site has a strict confidentiality policy.

KudoZ™ translation help

The KudoZ network provides a framework for translators and others to assist each other with translations or explanations of terms and short phrases.


See also:
Term search
  • All of ProZ.com
  • Term search
  • Jobs
  • Forums
  • Multiple search