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  3. Biology (-tech,-chem,micro-)

charged

Russian translation: заполняются (загружаются) коэлентеразином

16:26 Mar 20, 2020
English to Russian translations [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: charged
In certain embodiments of this invention, biosensor cells that produce aequorin as the reporter protein are charged with coelenterazine (CTZ) prior to their use in a detection assay. This charging step covalently links the aequorin to a hydrophobic prosthetic group (e.g., CTZ) and upon calcium (Ca2+) binding, the CTZ undergoes an irreversible reaction that includes a conformation change, and emits blue light (at 469 nm).

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Перед этим сказано, что клетки модифицируют, чтобы они вырабатывали экворин.

Из Википедии:
Cultured cells expressing the aequorin gene can effectively synthesize apoaequorin: however, recombinant expression yields only the apoprotein, therefore it is necessary to add coelenterazine into the culture medium of the cells to obtain a functional protein and thus use its blue light emission to measure Ca2+ concentration.
https://en.wikipedia.org/wiki/Aequorin#Applications
lerethel
Russian Federation
Local time: 03:55
Russian translation:заполняются (загружаются) коэлентеразином
Explanation:
***
Selected response from:

Marlin31
Russian Federation
Local time: 03:55
Grading comment
4 KudoZ points were awarded for this answer



Summary of answers provided
3 +1заполняются (загружаются) коэлентеразином
Marlin31
3см.
Igor Andreev
3 -1насыщаются
Vladyslav Golovaty


  

Answers


14 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5 peer agreement (net): +1
заполняются (загружаются) коэлентеразином


Explanation:
***

Marlin31
Russian Federation
Local time: 03:55
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 262

Peer comments on this answer (and responses from the answerer)
agree  Natalie: Обычно говорят, что "клетки нагружают"
2 hrs
  -> Спасибо, Наталья!
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54 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5 peer agreement (net): -1
насыщаются


Explanation:


Vladyslav Golovaty
Ukraine
Local time: 03:55
Works in field
Native speaker of: Native in EnglishEnglish, Native in RussianRussian
PRO pts in category: 44

Peer comments on this answer (and responses from the answerer)
disagree  Natalie: Зачем сочинять?
2 hrs
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4 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5
см.


Explanation:
перефразировать:
... клетки, которые продуцируют ..., инкубируют с целентеразином/в присутствии целентеразина. На этом этапе происходит образование ковалентной связи между экворином и простетической группой...

Igor Andreev
Local time: 03:55
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 1427
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