Electroshock conversion

Russian translation: электропорация

17:50 Apr 25, 2020

English to Russian translations [PRO]
Medical - Medical: Pharmaceuticals

English term or phrase: Electroshock conversion

The construction of a high yield stable cell line is briefly described as follows: host cells were grown in suspension in CD-CHO medium (Gibco, CA), the host cells in logarithmic growth phase were centrifuged, resuspended in fresh CD-CHO medium, the cells were counted and the cell density was adjusted to 1.43×107 cells/ml, 600ul of the above cell suspension was added to an electroporation cuvette, and then 40ug linearized plasmid was added, and pipetting was used to mix the cells with the plasmid uniformly.
*Electroshock conversion* was performed using a Bio-rad electrometer with instrument parameters set as follows: capacitance:
960uFD, and voltage:
300 V. Typically the electric shock are performed for 15-20 milliseconds, which are normal.
The electrically shocked cells were immediately resuspended in 37 °C pre-heated CD-CHO medium, inoculated in a 96-well plate at 100ul per well, and 2-3 days later an equal amount of screening medium (CD-CHO media + 50 uM MSX) was added.

Maxim Polukhin KudoZ activity Questions: 8838 (2 open) (1 without valid answers) (739 closed without grading) Answers: 43
Local time: 08:22

Russian translation:электропорация

Explanation:
Electroshock conversion was performed = Электропорацию осуществляли

Мда, ну однако товарищи китайцы и пишут :-)

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Natalie
Poland
Local time: 07:22

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электропорация

Natalie

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electroshock conversion

электропорация

Explanation:
Electroshock conversion was performed = Электропорацию осуществляли

Мда, ну однако товарищи китайцы и пишут :-)

Natalie
Poland
Local time: 07:22
Specializes in field
Native speaker of: Russian
PRO pts in category: 13399

Grading comment

Спасибо!

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Electroshock conversion

Russian translation: электропорация

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Russian translation: электропорация

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