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templated

English translation: specified by the DNA template

14:11 Jul 5, 2013
English language (monolingual) [PRO]
Science - Biology (-tech,-chem,micro-) / Biochemistry
English term or phrase: templated
Dear Colleagues,

I'd be very grateful if you could shed some light on the meaning of the word 'templated' in the following context:

'The method most frequently used to make capped RNAs in vitro is to transcribe a DNA template with either a bacterial RNA polymerase or a bacteriophage RNA polymerase in the presence of all four ribonucleoside triphosphates and a cap dinucleotide such as m7G(5′)ppp(5′)G. The polymerase initiates transcription with a nucleophilic attack by the 3′-OH of the Guo moiety in m7GpppG on the α-phosphate of the next templated nucleoside triphosphate, resulting in the initial product m7GpppGpN. The alternative, GTP-initiated product pppGpN is suppressed by setting the ratio of m7GpppG to GTP between 5 and 10 in the transcription reaction mixture.'

Full version of the document is available at http://www.google.com/patents/US20060252115.

Thank you for your assistance.
K S (X)
United Kingdom
Local time: 18:56
Selected answer:specified by the DNA template
Explanation:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2390807/

There is lots of excellent detailed material online about the mechanism of action of RNA polymerase.
Selected response from:

Peter Nicholson (X)
Poland
Local time: 19:56
Grading comment
Thank you, Peter. This is exactly what I've been looking for.
4 KudoZ points were awarded for this answer



SUMMARY OF ALL EXPLANATIONS PROVIDED
5 +1specified by the DNA template
Peter Nicholson (X)


Discussion entries: 1





  

Answers


15 hrs   confidence: Answerer confidence 5/5 peer agreement (net): +1
specified by the DNA template


Explanation:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2390807/

There is lots of excellent detailed material online about the mechanism of action of RNA polymerase.


Peter Nicholson (X)
Poland
Local time: 19:56
Does not meet criteria
Native speaker of: English
PRO pts in category: 4
Grading comment
Thank you, Peter. This is exactly what I've been looking for.

Peer comments on this answer (and responses from the answerer)
agree  Polangmar: OK, this should do: http://tinyurl.com/lqj366v , http://tinyurl.com/ls9qoqa - Kowalska is the patent's co-author, Grudzień is often cited in it (but another, more detailed rewording would be welcome).
1 day 14 hrs
  -> Thank you
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