Schnittstellen

English translation: cuts, cutting points

20:35 Mar 12, 2010
German to English translations [PRO]
Science - Genetics
German term or phrase: Schnittstellen
Mit Hilfe der Gentechnik wird zudem versucht, die Frequenzen für eine homologe Rekombination in Pflanzen zu erhöhen, zum Beispiel durch die Expression von Enzymen, die an wenigen Stellen des pflanzlichen Genoms Schnitte einfügen, um dort die homologe Rekombination zu induzieren.
Ein besonders viel versprechender Ansatz ist die Integration von Schnittstellen für eine Rekombinase, wie sie auch für die Eliminierung von Markergenen genutzt wird.
EHI (X)
Local time: 16:06
English translation:cuts, cutting points
Explanation:
The point where an incision is made in the plant in order to introduce something into it.
Selected response from:

Tom Bodine
United States
Local time: 07:06
Grading comment
Thank you very much, Tom
4 KudoZ points were awarded for this answer



Summary of answers provided
3 +3cuts, cutting points
Tom Bodine
Summary of reference entries provided
Schnittstelle/cutting point (site)
Bernhard Sulzer

Discussion entries: 1





  

Answers


19 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5 peer agreement (net): +3
cuts, cutting points


Explanation:
The point where an incision is made in the plant in order to introduce something into it.

Tom Bodine
United States
Local time: 07:06
Native speaker of: English
PRO pts in category: 4
Grading comment
Thank you very much, Tom

Peer comments on this answer (and responses from the answerer)
agree  Bernhard Sulzer: also cutting sites, see my refs.
9 hrs

agree  Rolf Keiser
13 hrs

agree  Norbert Hermann
14 hrs
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Reference comments


9 hrs
Reference: Schnittstelle/cutting point (site)

Reference information:
http://www.bentham.org/cg/sample/cg1-1/Portin.pdf

These enzymes cut DNA molecules each at a
specific site. These observations made it possible to
isolate genes, to clone them and analyze their
biochemical structure in great detail. Following the
action of restriction endonucleases, there often arise
so-called cohesive ends in the DNA molecules [58],
which tend to join together. By this means it is
possible for example to join together DNA from
any eukaryotic organism and that from the bacterial
plasmids. Such recombinant DNA molecules were
first constructed by David Jackson et al. [59], Peter
Lobban and Armin Kaiser [60] and Stanley Cohen
et al. [61]. Cloned DNA molecules can be
physically mapped, using the cutting points of the
restriction endonucleases as markers, ...

http://www.gmo-safety.eu/en/gene_transfer/elimination/463.do...

precursor project, which has since been concluded, demonstrated that it is possible to excise a gene from the plant genome using the "I-SceI" cutting sites. The next step is to integrate this gene at a different, precisely defined position in the plant genome by homologous recombination. This requires a specific site of integration , or target locus, in which to insert the gene sequence, or targeting vector.

Bernhard Sulzer
United States
Native speaker of: German
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