dopisanie nici komplementarnej

English translation: adding the complementary strand

23:49 Jan 6, 2015
Polish to English translations [PRO]
Science - Biology (-tech,-chem,micro-) / Molecular Biology
Polish term or phrase: dopisanie nici komplementarnej
General Context:
Zagadnienie w ramach przedmiotu "biologia molekularna".

Precise Context:
Poznawanie budowy genów. Metody sekwencjonowania. Wykonanie odczytu sekwencji DNA, dopisanie nici komplementarnej.

Dziękuję z góry za pomoc.
Darius Saczuk
United States
Local time: 00:43
English translation:adding the complementary strand
Explanation:
Zazwyczaj sekwencje genów podaje się w postaci od 5' do 3', przedstawiając nić sensowną DNA (a nie matrycę) lub nić RNA, czyli w podanym przykładzie sekwencję UAC GAU UCG UAU(RNA) lub TAC GAT TCG TAT (DNA).

Czyli komplementarna do nici DNA - ATGGCTAGC będzie TACCGATCG. Z kolei komplementarna do nici RNA - AUGCCG będzie UACGGC. - http://pl.wikipedia.org/wiki/Zasada_komplementarności

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Since there is only one complementary base for any of the bases found in DNA and in RNA, enzymes can make a complementary strand from any single strand. This is needed for DNA replication.

The bases which are complementary are

A with T
C with G
For example, the complementary strand of the DNA sequence

A G T C A T G
is

T C A G T A C

http://simple.wikipedia.org/wiki/Complementarity_(molecular_...

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Note added at 44 mins (2015-01-07 00:33:14 GMT)
--------------------------------------------------

The frayed-end DNA resembles the branched structure of the strand transfer product but differs in that its flanking strands are not associated with respective complementary strands. Therefore, we examined the impact on Mu end cleavage activity of adding the complementary strand to one or both of the flanking DNA strands - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1964541/

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This demonstrates that the absence of activity in the single-stranded reactions could be reconstituted by adding the complementary strand to the reaction. These results shown above strongly suggest that the target of TRAS1 EN in vivo is the telomeric region forming double-stranded DNA. - http://mcb.asm.org/content/21/1/100.full

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więcej przykładów - http://goo.gl/KBy3j8
Selected response from:

geopiet
Grading comment
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Summary of answers provided
1 +1adding the complementary strand
geopiet


  

Answers


40 mins   confidence: Answerer confidence 1/5Answerer confidence 1/5 peer agreement (net): +1
adding the complementary strand


Explanation:
Zazwyczaj sekwencje genów podaje się w postaci od 5' do 3', przedstawiając nić sensowną DNA (a nie matrycę) lub nić RNA, czyli w podanym przykładzie sekwencję UAC GAU UCG UAU(RNA) lub TAC GAT TCG TAT (DNA).

Czyli komplementarna do nici DNA - ATGGCTAGC będzie TACCGATCG. Z kolei komplementarna do nici RNA - AUGCCG będzie UACGGC. - http://pl.wikipedia.org/wiki/Zasada_komplementarności

-----------

Since there is only one complementary base for any of the bases found in DNA and in RNA, enzymes can make a complementary strand from any single strand. This is needed for DNA replication.

The bases which are complementary are

A with T
C with G
For example, the complementary strand of the DNA sequence

A G T C A T G
is

T C A G T A C

http://simple.wikipedia.org/wiki/Complementarity_(molecular_...

----------------



--------------------------------------------------
Note added at 44 mins (2015-01-07 00:33:14 GMT)
--------------------------------------------------

The frayed-end DNA resembles the branched structure of the strand transfer product but differs in that its flanking strands are not associated with respective complementary strands. Therefore, we examined the impact on Mu end cleavage activity of adding the complementary strand to one or both of the flanking DNA strands - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1964541/

-------------

This demonstrates that the absence of activity in the single-stranded reactions could be reconstituted by adding the complementary strand to the reaction. These results shown above strongly suggest that the target of TRAS1 EN in vivo is the telomeric region forming double-stranded DNA. - http://mcb.asm.org/content/21/1/100.full

-----------

więcej przykładów - http://goo.gl/KBy3j8

geopiet
Native speaker of: Native in PolishPolish
PRO pts in category: 36
Grading comment
Dziękuję za pomoc.

Peer comments on this answer (and responses from the answerer)
agree  Jacek Kloskowski: Sounds good to me, possibly "addition of a complementary strand" would be better in this context
13 hrs
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