английский => русский Медицина Биология (биотехника, биохимия, микробиология) лабораторная диагностика

Product Code CK113
Description of test
The IBD ELISA kit will measure the amount of antibody to IBD in the serum of chickens. Microtitre plates have been pre-coated with inactivated IBD antigen. Chicken serum samples are diluted and added to the microtitre wells where any anti-IBD antibodies present will bind and form an antigen-antibody complex. Non specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti-IBD antibodies bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti-IBD antibody is present and the intensity is directly related to the amount of anti-IBD present in the sample.

Reagents provided
1. IBD Coated plates. Inactivated viral antigen on microtitre plates.
2. Conjugate reagent Anti-Chicken: Alkaline Phosphatase in Tris bufler with protein stabilisers, inert red dye and sodium azide preservative (0.1 % w/v).
3. Substrate tablets. PNPP (p-Nitrophenyl Phosphate) tablets to dissolve with Substrate buffer.
4. Substrate buffer reagent. Diethanolamine bufler with enzyme co-factors.
5. Stop solution. Sodium Hydroxide in Diethanolamine bufier.
6. Sample diluent reagent. Phosphate bufler with protein stabilisers and sodium azide preservative (0.2 % w/v).
7. Wash buffer. Powdered Phosphate Buffered Saline with Tween.
8. Negative control. Specific Pathogen Free serum in Phosphate buffer with protein stabilisers and sodium azide preservative (0.2 % w/v).
9. Positive control. Antibodies specific to IBD in Phosphate buffer with protein stabilisers and sodium azide preservative (0.2 % w/v).

Materials and equipment required (not provided with kit)
Precision pipettes and disposable tips
8 or 12 channel pipette/repeater pipette
Plastic tubes or dilution plate for sample dilution
Distilled or deionized water
Microtiter plate reader with 405 nm filter
Microtiter plate washer