Feb 10, 2014 09:49
10 yrs ago
2 viewers *
English term
Italy
EN>IT, DE>IT TRANSLATIONS
Native in: Italian 
Works in: English to Italian, German to Italian
designer binder
English to Italian
Science
Genetics
proteomica
Buongiorno,
in un progetto di ricerca UE, i ricercatori vogliono estendere il metodo della proteomica all'analisi della segnalazione cellulare e delle proteine collegate al cancro sviluppando nuovi reagenti di affinità che legano le proteine bersaglio.
Through development of reagents that bind proteins, the project will advance methodologies for proteome-related analysis of cell signalling and cancer-related proteins. To gain key facts on binding sites, mode of action and protein interactions, researchers will apply ***designer binders*** to proteins-of-interest.
Grazie
in un progetto di ricerca UE, i ricercatori vogliono estendere il metodo della proteomica all'analisi della segnalazione cellulare e delle proteine collegate al cancro sviluppando nuovi reagenti di affinità che legano le proteine bersaglio.
Through development of reagents that bind proteins, the project will advance methodologies for proteome-related analysis of cell signalling and cancer-related proteins. To gain key facts on binding sites, mode of action and protein interactions, researchers will apply ***designer binders*** to proteins-of-interest.
Grazie
Proposed translations
(Italian)
| 2 | (fattori) leganti di sintesi |
dandamesh
|
| 3 | profili per ligando/di |
PLR TRADUZIO (X)
|
Proposed translations
26 mins
Selected
(fattori) leganti di sintesi
partendo da designer drugs, direi così
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Note added at 2 hrs (2014-02-10 12:34:07 GMT)
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attraverso lo sviluppo di software dedicati disegnano un ipotetico legante deve avere le caratteristiche richieste, poi lo sintetizzano o lo cercano, potrebbe essere qualcosa come
"ligandi disegnati"
Laboratorio di Metodologie Avanzate in Chimica Farmaceuticahttps://www.yumpu.com/.../laboratorio-di-metodologie-avanzat...Copia cache... 1q41, 1vyw); 3. disegnare i ligandi; 4. preparare i ligandi disegnati per il docking; ... Preparare le proteine scaricate dal PDB per il docking (applicare lo stesso ...
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Note added at 2 hrs (2014-02-10 12:34:07 GMT)
--------------------------------------------------
attraverso lo sviluppo di software dedicati disegnano un ipotetico legante deve avere le caratteristiche richieste, poi lo sintetizzano o lo cercano, potrebbe essere qualcosa come
"ligandi disegnati"
Laboratorio di Metodologie Avanzate in Chimica Farmaceuticahttps://www.yumpu.com/.../laboratorio-di-metodologie-avanzat...Copia cache... 1q41, 1vyw); 3. disegnare i ligandi; 4. preparare i ligandi disegnati per il docking; ... Preparare le proteine scaricate dal PDB per il docking (applicare lo stesso ...
4 KudoZ points awarded for this answer.
Comment: "grazie"
1 hr
profili per ligando/di
http://sbkb.org/update/research/designer-binders
Designer binders SBKB [doi:10.1038/nmeth.2719] Researchers define rational design principles to make a small-molecule ligand-binding protein from scratch. Small-molecule ligand-binding proteins are important tools for biology and biotechnology. Such tools can be created by raising antibodies against the molecule of interest or by directed evolution of an existing protein with a low affinity to the ligand to increase its binding power. But neither of these established approaches offers complete design flexibility or complete control over the binding interaction. David Baker of the University of Washington and his colleagues recently established principles guiding the rational design of ligand-binding proteins. They identified three crucial properties for creating a successful designer binder: the existence of specific hydrogen bonding and van der Waals interactions between the protein binding site and ligand, good shape complementarity between the binding site and ligand, and a structurally 'preorganized' binding site. They used computational tools to design an optimal binding site for the steroid ligand digoxigenin, positioning appropriate amino acid side chains around it to satisfy the three guiding design principles. They stationed the designed binding site in a set of receptive protein scaffold structures. To increase binding affinity, they further computationally optimized the residues surrounding the binding site. Then they evaluated the designs for binding affinity, shape complementarity and binding-site preorganization, and they selected 17 promising sequences for experimental characterization. The researchers displayed the 17 designer binders on the surface of yeast and panned for binding to digoxigenin by flow cytometry. Of the 17, 2 bound digoxigenin, which the team confirmed by fluorescence polarization and isothermal titration calorimetry. Not stopping there, they optimized the top binder using site-saturation mutagenesis and yeast surface-display selection. Further, they used deep sequencing to map the effects on binding of single amino acid mutations at crucial positions. Implementing these results, they finally attained a highly specific binder with picomolar affinity for digoxigenin, a degree of affinity typically characteristic of antibodies. Not only does the work offer new insights into protein-ligand interaction chemistry, the digoxigenin designer binder may also be a useful therapeutic tool. Whether the approach can be used to create other useful designer binders remains to be seen, but the outlook seems promising indeed. Allison Doerr
Read more at: http://sbkb.org/update/research/designer-binders
I recettori cellulari - Informasalus.it
www.informasalus.it/it/articoli/recettori-cellulari.php
07/ago/2012 - Viene definito recettore qualsiasi proteina che lega una specifica molecola segnale extracellulare (ligando) e innesca una risposta cellulare.
[PDF]
LA SEGNALAZIONE CELLULARE - Facoltà di Medicina e Chirurgia
www.medicina.unifg.it/.../05_SEGNALAZIONE CELLULARE.pdf
traslocazione di proteine da un compartimento cell. ad un altro, ecc. • I segnali ... degradati insieme con i loro ligandi e la cellula raggiunge uno stato di minore ...
[PDF]
Metodi per lo Studio dell'Equilibrio Chimico
biochimica.bio.uniroma1.it/didattica/dottorato/phd_eq.pdf
E' frequente che la funzione di una proteina (ad es. un enzima; un recettore; un trasportatore) comporti ... Se l'affinita' della proteina per il ligando e' alta ed il.
[PDF]
MIOGLOBINA E EMOGLOBINA
users.unimi.it/biolstru/teaching/Mioglobina.pdf
Interazioni proteina-ligando come base della funzione di molte proteine ... delle Proteine. - la maggior parte delle proteine possiede un'attività biologica che può.
[PDF]
studi strutturali e identificazione di ligandi della proteina ... - FedOA
www.fedoa.unina.it/3477/1/Tesi_Farina_B.pdf
di B Farina - 2008 - Articoli correlati
4. Risultati e discussione: identificazione di ligandi della proteina PED. 4.1. Allestimento e caratterizzazione della library di piccole molecole organiche. 74. 4.2
Designer binders SBKB [doi:10.1038/nmeth.2719] Researchers define rational design principles to make a small-molecule ligand-binding protein from scratch. Small-molecule ligand-binding proteins are important tools for biology and biotechnology. Such tools can be created by raising antibodies against the molecule of interest or by directed evolution of an existing protein with a low affinity to the ligand to increase its binding power. But neither of these established approaches offers complete design flexibility or complete control over the binding interaction. David Baker of the University of Washington and his colleagues recently established principles guiding the rational design of ligand-binding proteins. They identified three crucial properties for creating a successful designer binder: the existence of specific hydrogen bonding and van der Waals interactions between the protein binding site and ligand, good shape complementarity between the binding site and ligand, and a structurally 'preorganized' binding site. They used computational tools to design an optimal binding site for the steroid ligand digoxigenin, positioning appropriate amino acid side chains around it to satisfy the three guiding design principles. They stationed the designed binding site in a set of receptive protein scaffold structures. To increase binding affinity, they further computationally optimized the residues surrounding the binding site. Then they evaluated the designs for binding affinity, shape complementarity and binding-site preorganization, and they selected 17 promising sequences for experimental characterization. The researchers displayed the 17 designer binders on the surface of yeast and panned for binding to digoxigenin by flow cytometry. Of the 17, 2 bound digoxigenin, which the team confirmed by fluorescence polarization and isothermal titration calorimetry. Not stopping there, they optimized the top binder using site-saturation mutagenesis and yeast surface-display selection. Further, they used deep sequencing to map the effects on binding of single amino acid mutations at crucial positions. Implementing these results, they finally attained a highly specific binder with picomolar affinity for digoxigenin, a degree of affinity typically characteristic of antibodies. Not only does the work offer new insights into protein-ligand interaction chemistry, the digoxigenin designer binder may also be a useful therapeutic tool. Whether the approach can be used to create other useful designer binders remains to be seen, but the outlook seems promising indeed. Allison Doerr
Read more at: http://sbkb.org/update/research/designer-binders
I recettori cellulari - Informasalus.it
www.informasalus.it/it/articoli/recettori-cellulari.php
07/ago/2012 - Viene definito recettore qualsiasi proteina che lega una specifica molecola segnale extracellulare (ligando) e innesca una risposta cellulare.
[PDF]
LA SEGNALAZIONE CELLULARE - Facoltà di Medicina e Chirurgia
www.medicina.unifg.it/.../05_SEGNALAZIONE CELLULARE.pdf
traslocazione di proteine da un compartimento cell. ad un altro, ecc. • I segnali ... degradati insieme con i loro ligandi e la cellula raggiunge uno stato di minore ...
[PDF]
Metodi per lo Studio dell'Equilibrio Chimico
biochimica.bio.uniroma1.it/didattica/dottorato/phd_eq.pdf
E' frequente che la funzione di una proteina (ad es. un enzima; un recettore; un trasportatore) comporti ... Se l'affinita' della proteina per il ligando e' alta ed il.
[PDF]
MIOGLOBINA E EMOGLOBINA
users.unimi.it/biolstru/teaching/Mioglobina.pdf
Interazioni proteina-ligando come base della funzione di molte proteine ... delle Proteine. - la maggior parte delle proteine possiede un'attività biologica che può.
[PDF]
studi strutturali e identificazione di ligandi della proteina ... - FedOA
www.fedoa.unina.it/3477/1/Tesi_Farina_B.pdf
di B Farina - 2008 - Articoli correlati
4. Risultati e discussione: identificazione di ligandi della proteina PED. 4.1. Allestimento e caratterizzazione della library di piccole molecole organiche. 74. 4.2
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