This unit describes techniques for the preparation of mouse cerebellar slices - https://www.semanticscholar.org/paper/Recording-in-the-cereb...

-----

Figure 6.18.1

A schematic representation of excitatory synaptic pathways in the sagittal cerebellar slice preparation. - https://isn.page.link/Mx65

------

2.1 Preparation of the cerebellum

Because of its relatively small size, cerebellar slices can be prepared using a vibratome or McIlwain tissue chopper (Lonchamp et al., 2006). We have selected the vibratome method for consistency in sectioning. Cerebellar cultures can be prepared in sagittal and transversal sections and demonstrate excellent viability with both orientations. - https://www.sciencedirect.com/topics/medicine-and-dentistry/...

-----

Figure 7.2. Preparation of sagittal cerebellar slices.

(A) The brain is trimmed according to the dashed lines, and the cerebellum is bisected at the midline.
(B) The chuck is oriented in the middle of the specimen disc for sectioning.
(C) Low magnification image of a cerebellar slice cultured for 3 weeks and labeled with anti-Calbindin D28K to highlight the Purkinje cells.
(D) High magnification image of C showing dense dendritic arborization in the molecular layer.
(E) Representative morphology of mature Purkinje neurons, showing extensive dendritic shafts and terminals.
(F) Magnified image of the boxed region in E, showing numerous dendritic spines in culture. Scale bar is 250 μm for C and D; 10 μm for E and F.
- https://isn.page.link/KtbJ