After demonstrating bulk gene expression for our four-plex assay TERT, MET, VIM, and ALDH, we observed distinct patterns of gene expression on individual cells isolated from doped blood (Fig. 3B).

Four-Plex Gene Expression of Single H1650 Cells Spiked into Healthy Blood.
To validate the suitability of a modular gene panel including vimentin (VIM) and aldehyde dehydrogenase (ALDH) in addition to TERT and MET for CTC identification, we examined the bulk gene expression of 106 cells from each of five NSCLC cell lines (A549, H661, H1650, H1975, and HCC827), a noncancerous fibroblast (PCS-201) cell line, and WBCs from 2 mL of healthy human blood. We noted heterogeneous VIM and ALDH expression across these cell types (Fig. S3).
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5206556/


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Note added at 8 hrs (2019-06-23 00:50:16 GMT)
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PCR = polymerase chain reaction
Singleplex PCR is used to detect one target sequence of DNA or RNA (as shown in the upper image). This assay could be used to detect a specific virus or bacteria or determine if an individual has a gene of interest.
https://www.medialabinc.net/courses/imgs/40457-631020_164304...
Multilplex PCR is used to detect two or more target sequences of DNA or RNA simultaneously (shown in the lower image) using a single sample preparation and amplification. Multiple sets of primers and probes are included to allow more targets/analytes to be detected in a single run.
https://www.medialabinc.net/courses/imgs/40457-631021_164304...
https://www.labce.com/spg1001965_singleplex_versus_multiplex...