English to Italian Science Biology (-tech,-chem,micro-)

This is in an article about "Antibiofilm efficacy of tea tree oil and of its main component terpinen- 4-ol against Candida albicans"


Biofilm formation on denture base resin specimens and ***mouthwash simulation***

Denture base acrylic resin was chosen for this study (Vipi Colorless Wave).
The final dimensions of the disc-shaped acrylic resin patterns were 10 mm in diameter and 2 mm in thickness.
The surface roughness of specimens was measured (0.2 µm) (Mitutoyo Surftest SJ-401, Mitutoyo Corporation, Japan).
Distribution between experimental and control groups was determined by randomization and restriction.
Prior to the experiment, the specimens were sterilized with ethylene oxide.
The assays for adhesion and biofilm formation were performed according to the literature.17 Each denture base acrylic resin specimen was placed in sterile 24-well flat-bottomed microtiter plates.
Then, 1 mL of human saliva was added, incubated for 1 h at 37ºC, and shaken at 75 rpm to allow salivary pellicle formation on the specimens.18 Human unstimulated whole saliva (Certificate of Presentation for Ethical Consideration - CAAE 06687412.5.0000.5416) was collected and clarified by centrifugation at 3,800 g for 10 min at 4°C. The supernatant was sterilized by filtration (22 m) and stored at -70° C until use.
After incubation, C. albicans was dispensed into
each well and the plates were incubated at 37°C for 90 min (adhesion assay) at 75 rpm and for 72 h. The culture medium was renewed every 24 h during biofilm formation.
After biofilm formation, each specimen was treated with 1 mL of the solutions for 60 s, simulating the clinical application of mouthwash:
17.92 mg/mL of TTO, 8.86 mg/mL of terpinen-4-ol, 256 g/mL of nystatin and phosphate-buffered saline.
Subsequently, residual biofilms were disrupted and adhered microorganisms were removed from the specimens by sonication (7 W for 20 s)19 and then washed with PBS.