Rekombinant antistoff, anvendelse, fremgangsmate for fremstilling, nukleinsyre, vektor, vertcelle samt farmasoytisk preparat Classifications (Patent NO328687B1)

Recombinant antibodies give you the highest level of consistency between batches, peace of mind with an uninterrupted supply, and the ability to engineer sensitivity and specificity. ​​Monoclonal antibodies are typically made using B-cells from an immunized animal to form immortal hybridoma cells that secrete the desired antibody clone. This hybridoma technique produces highly consistent, specific and sensitive monoclonal antibodies in large quantities. However, over time, hybridoma cell lines can experience genetic drift, resulting in slight variations to the antibodies produced. There is also a growing demand for antibodies against difficult targets, ie toxins, nucleotides, and membrane-bound proteins, that can’t always be made with this in vivo model. (Abcam)

Recombinant antibodies (rAbs) are produced by cloning antibody genes into expression vectors, which are subsequently introduced into expression hosts to provide animal-free antibody production. By employing genetic engineering methods, it is possible to express light and heavy chains of immunoglobulins as individual proteins, and create a whole set of antibody fragments with improved affinity, stability, and the desired spatial orientation of antigen binding sites or modify potential immunogenicity. The ability to manipulate the genes that encode antibodies (Ab), and thereby manipulate the structures of antibodies, has opened a new era in the study and application of antibodies. (Enzo Life Sciences)